Inhibitive Properties of Anredera Cordifolia Extract on Escherichia Coli Swimming Motility

Alexander Tobias Kristanto, Maria Silvia Merry, Suryani Hutomo

Abstract


Background Escherichia coli, a Gram-negative bacterium, is one of the main etiology of urinary tract infection (UTI). An important pathogenicity factor influencing biofilm production is its motility, swimming, or swarming. Anredera cordifolia (AC) leaves have been proven to have an antibacterial effect on various Gram-positive and Gram-negative bacteria, including E. coli.

Objective To determine the effect of AC leaves extract on swimming motility property of E. coli derived from UTI patients. Methods AC leaves were extracted with the maceration method. E. coli was identified and isolated from UTI patients. There are

4 diluting concentrations of AC leaves extract (from 875 mg per ml to 7000 mg per ml) and one negative control. The preparation underwent incubation, inoculation, and re-incubation. Swimming motility was measured manually, and the result was tested for significant difference between each dose with one-way ANOVA (limit of significance is 0.05)

Results On AC leaves extract concentration of 1750 mg per ml, the swimming motility has been inhibited significantly compared to negative control and lower concentration of 875 mg per ml (0.543 cm vs 1.673 cm and 1.358 cm, respectively; p < 0.001). Increasing concentration (3500 mg per ml and 7000 mg per ml) did not show a significant difference in inhibition compared to 1750 mg per ml (0.539 cm and 0.530 vs 0.543).

Conclusion AC leaves extract with a concentration of at least 1750 mg per ml would inhibit the swimming motility of E. Coli derived from UTI patients.

Keywords


Escherichia coli; Anredera cordifolia; swimming motility; urinary tract infections

Full Text:

PDF

References


Duan Q, Zhou M, Zhu L, Zhu G. Flagella and bacterial pathogenicity. J Basic Microbiol. 2013;53(1):1–8.

Riedel S, Hobden JA, Miller S, Morse SA, Mietzner TA, Detrick B, et al. Enteric Gram- Negative Rods (Enterobacteriaceae). In: Jawetz, Melnick, & Adelberg’s Medical Microbiology, 28e [Internet]. New York, NY: McGraw-Hill Education; 2019 [cited 2022 Feb 28]. Available from: accessmedicine.mhmedical.com/content.aspx?ai d=1163279866

Prabowo FI, Habib I. Identifikasi Pola Kepekaan dan Jenis Bakteri pada Pasien Infeksi Saluran Kemih di Rumah Sakit PKU Muhammadiyah Yogyakarta. Mutiara Med. 2012;12(2):93–101.

Sugianli AK, Ginting F, Kusumawati RL, Parwati I, de Jong MD, van Leth F, et al. Laboratory- based versus population-based surveillance of antimicrobial resistance to inform empirical treatment for suspected urinary tract infection in Indonesia. PloS One. 2020 Mar 30;15(3):e0230489–e0230489.

Leliqia NPE, Sukandar EY, Fidrianny I. Overview of efficacy, safety and phytochemical study of Anredera cordifolia(Ten.) steenis. Pharmacol Online. 2017;1:124–31.

Alba TM, de Pelegrin CMG, Sobottka AM. Ethnobotany, ecology, pharmacology, and chemistry of Anredera cordifolia (Basellaceae): a review. Rodriguésia. 2020;7.

Paju N, Yamlean PVY, Kojong N. Uji efektivitas salep ekstrak daun binahong (Anredera cordifolia (Ten.) Steenis) pada kelinci (Oryctolagus cuniculus) yang terinfeksi bakteri Staphylococcus aureus. Pharmacon J Ilm Farm. 2013;2(1):51–61.

Rimporok S, Kepel BJ, Siagian KV. Uji efektivitas ekstrak daun binahong (Anredera cordifolia Steenis) terhadap pertumbuhan Streptococcus mutans secara in vitro. Pharmacon J Ilm Farm. 2015;4(4):15–21.

Sari MK, Tumangger BS, Sorbakti BJ, Helmalia F, Fadhliani. Effectiveness of the binahong leaf extract (Anredera cordifolia) in devoting bacterial growth Vibrio cholerae in vitro. In Bristol: IOP Publishing; 2020. p. 12073.

Moat AG, Foster JW, Spector MP. Microbial Physiology. Hoboken: John Wiley & Sons, Incorporated; 2002.

Darsana IGO, Besung INK, Mahatmi H. Potensi Daun Binahong (Anredera Cordifolia (Tenore) Steenis)dalam Menghambat Pertumbuhan Bakteri Escherichia Coli secara In Vitro. Indones Med Veterinus. 2012;1(3):337–51.

Budianto B, Arumsari AG, Inayah N, Fatmaningrum F, Suparmi A. Comparison of the effectiveness of leaf extracts of anredera cordifolia, psidium guajava and pogostemon cablin on inhibitory power over escherichia coli bacteria. Vitae Medellín. 2021;28(3).

Minami M, Takase H, Nakamura M, Makino T. Methanol extract of Lonicera caerulea var. emphyllocalyx fruit has anti-motility and anti- biofilm activity against enteropathogenic Escherichia coli. Drug Discov Ther. 2019;13(6):335–42.

Magnini RD, Nitiéma M, Ouédraogo GG, Ilboudo S, Bancé A, Millogo-Koné H, et al. Toxicity and bacterial anti-motility activities of the hydroethanolic extract of Acacia senegal (L.) Willd (Fabaceae) leaves. BMC Complement Med Ther. 2021 Jun 29;21(1):178–178.




DOI: http://dx.doi.org/10.21460/bikdw.v6i2.304

Refbacks

  • There are currently no refbacks.


Copyright (c) 2022 Berkala Ilmiah Kedokteran Duta Wacana

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.