Yustina Nuke Ardiyan


Background: Tissue staining methods need controls to give best quality results. Controls is necessary to support the validity, correctness, and reliability of the staining results. Haematoxylin and Eosin (HE) and immunohistochemistry (IHC) staining require different control quality.
Objective: To discuss the utilization of controls used in tissue staining.
Method: This is a narrative review with literature from 2008-2016.
Results: Routine staining usually does not require any control. Specific stainings require controls to support the validity, correctness, and reliability of the staining results. Histochemistry staining requires only positive control, while positive and negative controls must be included in IHC staining. A tissue section which expresses the protein of interest is reffered to positive control, whereas those without any target antigen expression are defined as negative control.
Conclusion: Histochemistry and IHC staining controls are needed to obtain the validity of the tissue or cell samples.


immunohistochemistry, quality control, review

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Ovalle WK, Nahirney PC. Netter’s Essential Histology. 2nd ed. Philadelphia: Elsevier; 2013. Appendix, Staining methods and technique; p. 479-80.

Mescher AL. Junqueira’s Basic Histology Text and Atlas. 12th ed. New York: McGraw-Hill Medical; 2010.

Otali D, Fredenburgh J, Oelschlager DK, Grizzle WE. A standard tissue as a control for histochemistry and immune-histochemistry staining. Biotech and Histochem. 2016; 91(5): 309–26.

Hewitt SM, Baskin DG, Frevert CW, Stahl WL, Rosa-Molinar E. Controls for immunohisto-chemistry: The histochemistry society’s standards of practice for validation of immunohisto-chemistry assays. Journal of Histochemistry and Cytochemistry. 2014; 62(10): 693–7.

Burry RW. Controls for immunocytochemistry: An update. Journal of Histochemistry and Cyto-chemistry. 2011; 59(1): 6–12.

Sanderson T, Wild G, Michelle A, Jennifer C, Greg M. Immuno-histochemistry and immune-fluorescent techniques. In: Suvarna L, Christopher JD. Bancroft: Bancroft's Theory and Practice of Histological Techniques. 8th ed. Philadelphia: Elsevier, 2018:337-94.

Fischer AH, Jacobson KA, Rose J, Zeller R. Hematoxylin and eosin staining of tissue and cell sections. Cold Spring Harbor Protocols 2008; 3(5): 4986–8.

Torlakovic EE, Nielsen S, Francis G, Garrat J, Gilks B, Goldsmith JD, Hornick J, Hyjek E, Ibrahim M, Miller K, Petcu E, Swanson PE, Zhou X, Taylor CR, Vyberg M. Standardization of Negatif Kontrols in Diagnostic Immunohistochemistry: Recommendations from the International Ad Hoc Expert Panel. Appl Immunohistochem Mol Morphol. 2015; 23(1): 1–18.

Qureshi A, Pervez S. Allred scoring for ER reporting and its impact in clearly distinguishing ER negative from ER positive breast cancers. Journal of the Pakistan Medical Association. 2010; 60(5): 350–3.

H&E stains of normal tissue, special stains, immuno histochemistry stain. Available from: URL: http://www.ihcworld. com

Campbell EJ, Tesson M, Doogan F, Mohammed ZMA, Mallon E, Edwards J. The combined endocrine receptor in breast cancer, a novel approach to traditional hormone receptor interpretation and a better discriminator of outcome than ER and PR alone. British Journal of Cancer. 2016; 115(8): 967-973.

DOI: http://dx.doi.org/10.21460/bikdw.v5i2.180


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