IMMUNOPROTECTIVE EFFECT OF MELATONIN ADMINISTRATION ON METHYLPREDNISOLONE-TREATED WISTAR MALE RAT (RATTUS NORVEGICUS)

Widya Christine Manus, Wimpie Pangkahila, I Gusti Made Aman

Abstract


Background: Immune system function decreases with age. Steroid use might accelerate this process, especially in adults in Indonesia. Melatonin is a natural hormone which show antioxidant effect and immunoprotective effect. Melatonin administration might help in reviving humane immune system due to aging and exogenous steroid exposure.
Objective: This experiment is aimed to determine the effect of melatonin on leukocyte and lymphocyte count of methylprednisolone-induced immunosuppression on Wistar rat.
Method: This study was true experimental with post-test only control group design using 32 male rats. Rats were divided into control and the intervention group. Control group was given methylprednisolone 0.144 mg/200 g rat 3 times a day and distilled water. The treatment group was given methylprednisolone 0.144/200 g rat 3 times a day and 0.054 mg/200 g melatonin once daily. The blood was taken after 14 days of treatment.
Results: Intervention group shows higher lymphocytes count (4.95 ±1.58x103/L) compared to the control group (3.091.33x103/L), with p < 0.01. The intervention group also has higher leukocyte count (8.37± 2.02x103/L) compared to the control group (6.70 ± 1.96x103/L), with p<0.05.
Conclusion: This study concluded that oral melatonin inhibited the decrease number of lymphocytes and leukocytes in rats given methylprednisolone.

Keywords


melatonin, leukocytes, lymphocytes, methylprednisolone, Wistar rats

Full Text:

PDF

References


Desai A, Grolleau-Julius A, Yung R. Leukocyte function in the aging immune system. J Leukoc Biol. 2010;87(6):1001-1009.

Coutinho AE, Chapman KE. The anti-inflammatory and immunosuppressive effects of glucocorticoids, recent developments and mechanistic insights. Mol Cell Endocrinol. 2011;335(1):2-13.

Pangkahila W. Anti aging: tetap muda dan sehat. Jakarta: Penerbit buku Kompas; 2011.

Pangkahila W, Wih WL. Evidence-based anti aging medicine hormone replacement therapy. Jakarta: TLC Publisher; 2015.

Hrapkiewicz K, Colby L, Denison P. Clinical laboratory animal medicine: an introduction. Oxford: Wiley-Blackwell; 2013.

Barnes PJ, Adcock IM. Glucocorticoid resistance in inflammatory diseases. Lancet. 2009;373(9678):1905-1917.

Rhen T, Cidlowski JA. Antiinflammatory action of glucocorticoids--new mechanisms for old drugs. N Engl J Med. 2005;353(16):1711-1723.

Cain DW, Cidlowski JA. Immune regulation by glucocorticoids. Nat Rev Immunol. 2017;17(4):233-247.

Davis TE, Kis-Toth K, Szanto A, Tsokos GC. Glucocorticoids suppress T cell function by up-regulating microRNA-98. Arthritis Rheum. 2013;65(7):1882-1890.

Torres RL, Torres ILdS, Laste G, Ferreira MBC, Cardoso PFG, Belló-Klein A. Effects of acute and chronic administration of methylprednisolone on oxidative stress in rat lungs. J Bras Pneumol. 2014;40(3):238-243.

Zen M, Canova M, Campana C, et al. The kaleidoscope of glucorticoid effects on immune system. Autoimmun Rev. 2011;10(6):305-310.

Kresno SB. Imunologi: Diagnosis dan Prosedur Laboratorium. 5 ed. Jakarta: Balai Penerbit FKUI; 2013.

Chiossone L, Vitale C, Cottalasso F, et al. Molecular analysis of the methylprednisolone-mediated inhibition of NK-cell function: evidence for different susceptibility of IL-2- versus IL-15-activated NK cells. Blood. 2007;109(9):3767-3775.

Carrillo-Vico A, Lardone PJ, Alvarez-Sánchez N, Rodríguez-Rodríguez A, Guerrero JM. Melatonin: buffering the immune system. Int J Mol Sci. 2013;14(4):8638-8683.

Espino J, Pariente JA, Rodríguez AB. Oxidative stress and immunosenescence: therapeutic effects of melatonin. Oxid Med Cell Longev. 2012;2012:670294-670294.

Baratawidjaja KG, Rengganis I. Imunologi Dasar. Jakarta: Balai Penerbit FKUI; 2009.




DOI: http://dx.doi.org/10.21460/bikdw.v4i2.150

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Berkala Ilmiah Kedokteran Duta Wacana

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.