IMMUNOPROTECTIVE EFFECT OF MELATONIN ADMINISTRATION ON METHYLPREDNISOLONE-TREATED WISTAR MALE RAT (RATTUS NORVEGICUS)

Authors

  • Widya Christine Manus Fakultas Kedokteran Universitas Udayana, Indonesia
  • Wimpie Pangkahila Universitas Udayana
  • I Gusti Made Aman Universitas Udayana, Indonesia

DOI:

https://doi.org/10.21460/bikdw.v4i2.150

Keywords:

melatonin, leukocytes, lymphocytes, methylprednisolone, Wistar rats

Abstract

Background: Immune system function decreases with age. Steroid use might accelerate this process, especially in adults in Indonesia. Melatonin is a natural hormone which show antioxidant effect and immunoprotective effect. Melatonin administration might help in reviving humane immune system due to aging and exogenous steroid exposure. Objective: This experiment is aimed to determine the effect of melatonin on leukocyte and lymphocyte count of methylprednisolone-induced immunosuppression on Wistar rat. Method: This study was true experimental with post-test only control group design using 32 male rats. Rats were divided into control and the intervention group. Control group was given methylprednisolone 0.144 mg/200 g rat 3 times a day and distilled water. The treatment group was given methylprednisolone 0.144/200 g rat 3 times a day and 0.054 mg/200 g melatonin once daily. The blood was taken after 14 days of treatment. Results: Intervention group shows higher lymphocytes count (4.95 ±1.58x103/ï­L) compared to the control group (3.09ï­1.33x103/ï­L), with p < 0.01. The intervention group also has higher leukocyte count (8.37± 2.02x103/ï­L) compared to the control group (6.70 ± 1.96x103/ï­L), with p<0.05. Conclusion: This study concluded that oral melatonin inhibited the decrease number of lymphocytes and leukocytes in rats given methylprednisolone.

Author Biographies

Widya Christine Manus, Fakultas Kedokteran Universitas Udayana

Program Magister Program Studi Ilmu Biomedik Kekhususan Anti Aging Program Pascasarjana

Wimpie Pangkahila, Universitas Udayana

Departemen Andrologi dan seksologi

I Gusti Made Aman, Universitas Udayana

Departemen Farmakologi

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Published

2019-12-27

Issue

Vol. 4 No. 2 (2019): BERKALA ILMIAH KEDOKTERAN DUTA WACANA

Section

Research

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